Screening for single-chain variable fragment antibodies against multiple Cry1 toxins from an immunized mouse phage display antibody library

Published: 14/03/2018
Screening for single-chain variable fragment antibodies against multiple Cry1 toxins from an immunized mouse phage display antibody library
Source: LINK.SPRINGER.COM

Abstract Single-chain variable fragment (scFv) is a kind of antibody that possess only one chain of the complete antibody while maintaining the antigen-specific binding abilities and can be expressed in prokaryotic system. In this study, scFvs against Cry1 toxins were screened out from an immunized mouse phage displayed antibody library, which was successfully constructed with capacity of 6.25 × 1

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