Engineering a thermostable highly active glucose 6-phosphate dehydrogenase and its application to hydrogen production in vitro

Published: 14/03/2018
Engineering a thermostable highly active glucose 6-phosphate dehydrogenase and its application to hydrogen production in vitro
Source: LINK.SPRINGER.COM

Abstract Glucose 6-phosphate dehydrogenase (G6PDH) is one of the most important dehydrogenases responsible for generating reduced NADPH for anabolism and is also the rate-limiting enzyme in the Entner-Doudoroff pathway. For in vitro biocatalysis, G6PDH must possess both high activity and good thermostability due to requirements of efficient use and low expense of biocatalyst. Here, we used directe

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